lady of the lake wrote:Hi y'all. I was wondering if anyone here might know how to mount nudibranch eggs on a glass slide?? I couldn't find any thread about microscopy, so I figured I'd just post here. Should I use a well slide??
Also, Is it possible to use a glass slide as a coverslip? I've tried is before, and it sort of worked, but it was very difficult to focus. Any tips??
I'm not too experienced using well slides (I'm use to looking at/staining microorganisms), but have you tried using dyes? Something as simple as iodine might make it easier to view the eggs cell components. Immersion oil might also help, but that depends on what magnification you plan on using (usually used to help focus on cells at 100x).
I wouldn't recommend using another glass slide as a coverslip, as they're usually too thick and it's hard to focus the microscope with one of them on top. But if that's all you've got, you might as well try! It could be easier with dye!
Edit: I have no idea the size of these eggs so if my methods seem weird with their size, feel free to correct me. Cx